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Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.

Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Klein, P G, Juneja, V K
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1997
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168763/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9361430/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.11.4441-4448.1997
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