Lanean...

Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR.

An in situ PCR protocol by which we can monitor the presence or absence of lac mRNA in individual cells of a Salmonella typhimurium F' lac+ strain has been developed. In this protocol, fixed cells are permeabilized with lysozyme and subjected to a seminested reverse transcriptase PCR using repo...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Tolker-Nielsen, T, Holmstrøm, K, Molin, S
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1997
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC168737/
https://ncbi.nlm.nih.gov/pubmed/9361404
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