Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR.
An in situ PCR protocol by which we can monitor the presence or absence of lac mRNA in individual cells of a Salmonella typhimurium F' lac+ strain has been developed. In this protocol, fixed cells are permeabilized with lysozyme and subjected to a seminested reverse transcriptase PCR using reporter...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9361404/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.11.4196-4203.1997 |
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