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In Situ Reverse Transcription-PCR for Monitoring Gene Expression in Individual Methanosarcina mazei S-6 Cells

An in situ reverse transcription-PCR protocol for detecting specific mRNA in Methanosarcina mazei S-6 is described. This method allowed us to detect heat shock-induced increases in the intracellular levels of the transcript of the universal stress gene dnaK. The cell walls of paraformaldehyde-fixed...

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主要な著者: Lange, Marianne, Tolker-Nielsen, Tim, Molin, Søren, Ahring, Birgitte K.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2000
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オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC101414/
https://ncbi.nlm.nih.gov/pubmed/10788341
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