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In Situ Reverse Transcription-PCR for Monitoring Gene Expression in Individual Methanosarcina mazei S-6 Cells

An in situ reverse transcription-PCR protocol for detecting specific mRNA in Methanosarcina mazei S-6 is described. This method allowed us to detect heat shock-induced increases in the intracellular levels of the transcript of the universal stress gene dnaK. The cell walls of paraformaldehyde-fixed...

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Udgivet i:Appl Environ Microbiol
Principais autores: Lange, Marianne, Tolker-Nielsen, Tim, Molin, Søren, Ahring, Birgitte K.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101414/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10788341/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.5.1796-1800.2000
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