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Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR.

An in situ PCR protocol by which we can monitor the presence or absence of lac mRNA in individual cells of a Salmonella typhimurium F' lac+ strain has been developed. In this protocol, fixed cells are permeabilized with lysozyme and subjected to a seminested reverse transcriptase PCR using repo...

詳細記述

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書誌詳細
主要な著者: Tolker-Nielsen, T, Holmstrøm, K, Molin, S
フォーマット: Artigo
言語:Inglês
出版事項: 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC168737/
https://ncbi.nlm.nih.gov/pubmed/9361404
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