Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR.

An in situ PCR protocol by which we can monitor the presence or absence of lac mRNA in individual cells of a Salmonella typhimurium F' lac+ strain has been developed. In this protocol, fixed cells are permeabilized with lysozyme and subjected to a seminested reverse transcriptase PCR using reporter...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Tolker-Nielsen, T, Holmstrøm, K, Molin, S
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9361404/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.11.4196-4203.1997
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