In Situ Reverse Transcription-PCR for Monitoring Gene Expression in Individual Methanosarcina mazei S-6 Cells
An in situ reverse transcription-PCR protocol for detecting specific mRNA in Methanosarcina mazei S-6 is described. This method allowed us to detect heat shock-induced increases in the intracellular levels of the transcript of the universal stress gene dnaK. The cell walls of paraformaldehyde-fixed...
שמור ב:
| הוצא לאור ב: | Appl Environ Microbiol |
|---|---|
| Principais autores: | , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
American Society for Microbiology (ASM)
2000
|
| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101414/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10788341/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.5.1796-1800.2000 |
| תגים: |
אין תגיות, היה/י הראשונ/ה לתייג את הרשומה!
|
