A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk.

A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial spores are concentrated by centrifugation after chemical extraction of the milk components. The vegetative cells are selectively lysed, and their DNA is digested and washed away. Af...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Herman, L M, De Block, J H, Waes, G M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167725/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8534081/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.12.4141-4146.1995
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