A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk.
A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial spores are concentrated by centrifugation after chemical extraction of the milk components. The vegetative cells are selectively lysed, and their DNA is digested and washed away. Af...
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| Publicado en: | Appl Environ Microbiol |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1995
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167725/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8534081/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.12.4141-4146.1995 |
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