A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk.

A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial spores are concentrated by centrifugation after chemical extraction of the milk components. The vegetative cells are selectively lysed, and their DNA is digested and washed away. Af...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Herman, L M, De Block, J H, Waes, G M
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1995
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167725/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8534081/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.12.4141-4146.1995
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