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Detection of a high-barrier conformational change in the active site of cytochrome P450(cam) upon binding of putidaredoxin

The orientation of the substrate camphor in the active site of reduced CO-bound cytochrome P450(cam) (CYP101) as a function of reduced putidaredoxin (Pdx(r)) addition has been examined by NMR using perdeuterated CYP101 and peredeuterated Pdx as well as isotopically labeled d-camphor. This permits th...

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Autors principals: Wei, Julie Y., Pochapsky, Thomas C., Pochapsky, Susan Sondej
Format: Artigo
Idioma:Inglês
Publicat: 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1661779/
https://ncbi.nlm.nih.gov/pubmed/15884940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja051195j
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