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Detection of a high-barrier conformational change in the active site of cytochrome P450(cam) upon binding of putidaredoxin
The orientation of the substrate camphor in the active site of reduced CO-bound cytochrome P450(cam) (CYP101) as a function of reduced putidaredoxin (Pdx(r)) addition has been examined by NMR using perdeuterated CYP101 and peredeuterated Pdx as well as isotopically labeled d-camphor. This permits th...
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| Main Authors: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2005
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1661779/ https://ncbi.nlm.nih.gov/pubmed/15884940 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja051195j |
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