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Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries

A method is presented for the preparation and use of fluorogenic peptide substrates that allows for the configuration of general substrate libraries to rapidly identify the primary and extended specificity of proteases. The substrates contain the fluorogenic leaving group 7-amino-4-carbamoylmethylco...

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Autori principali: Harris, Jennifer L., Backes, Bradley J., Leonetti, Francesco, Mahrus, Sami, Ellman, Jonathan A., Craik, Charles S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC16617/
https://ncbi.nlm.nih.gov/pubmed/10869434
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