Development of Reverse Transcription (RT)-PCR and Real-Time RT-PCR Assays for Rapid Detection and Quantification of Viable Yeasts and Molds Contaminating Yogurts and Pasteurized Food Products

Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds. Universal primers were designed based on the available fungal actin sequences, and by RT-PCR they amplified a specific 353-bp fragment from fungal species involved i...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Bleve, Gianluca, Rizzotti, Lucia, Dellaglio, Franco, Torriani, Sandra
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2003
Schlagworte:	Food Microbiology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC165170/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12839789/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.7.4116-4122.2003
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