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Optimization of Reverse Transcriptase PCR To Detect Viable Shiga-Toxin-Producing Escherichia coli

The ability of reverse transcriptase PCR (RT-PCR) to detect viable Shiga-toxin-producing Escherichia coli (STEC) was investigated. Four primer sets, each targeting a specific region in the slt-II operon, were evaluated for their stringency and specificity for slt-II mRNA. STEC were evaluated for tox...

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Udgivet i:Appl Environ Microbiol
Principais autores: McIngvale, S. C., Elhanafi, D., Drake, M. A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2002
Fag:
Food Microbiology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126677/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11823221/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.2.799-806.2002
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