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Optimization of Reverse Transcriptase PCR To Detect Viable Shiga-Toxin-Producing Escherichia coli

The ability of reverse transcriptase PCR (RT-PCR) to detect viable Shiga-toxin-producing Escherichia coli (STEC) was investigated. Four primer sets, each targeting a specific region in the slt-II operon, were evaluated for their stringency and specificity for slt-II mRNA. STEC were evaluated for tox...

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Detalhes bibliográficos
Main Authors: McIngvale, S. C., Elhanafi, D., Drake, M. A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC126677/
https://ncbi.nlm.nih.gov/pubmed/11823221
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.2.799-806.2002
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