Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

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Detalhes bibliográficos
Main Authors: Heller, Loree C., Davis, Carisa R., Kealy Peak, K., Wingfield, David, Cannons, Andrew C., Amuso, Philip T., Cattani, Jacqueline
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Food Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC150052/
https://ncbi.nlm.nih.gov/pubmed/12620880
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.3.1844-1846.2003
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