Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Podrobná bibliografie

Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Heller, Loree C., Davis, Carisa R., Kealy Peak, K., Wingfield, David, Cannons, Andrew C., Amuso, Philip T., Cattani, Jacqueline
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2003
Témata:	Food Microbiology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC150052/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12620880/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.3.1844-1846.2003
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