Structural and kinetic analyses of the H121A mutant of cholesterol oxidase

Cholesterol oxidase is a monomeric flavoenzyme that catalyses the oxidation of cholesterol to cholest-5-en-3-one followed by isomerization to cholest-4-en-3-one. The enzyme from Brevibacterium sterolicum contains the FAD cofactor covalently bound to His(121). It was previously demonstrated that the...

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Main Authors: Lim, Louis, Molla, Gianluca, Guinn, Nicole, Ghisla, Sandro, Pollegioni, Loredano, Vrielink, Alice
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2006
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635447/
https://ncbi.nlm.nih.gov/pubmed/16856877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20060664
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