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Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli

BACKGROUND: Cholesterol oxidase is an alcohol dehydrogenase/oxidase flavoprotein that catalyzes the dehydrogenation of C(3)-OH of cholesterol. It has two major biotechnological applications, i.e. in the determination of serum (and food) cholesterol levels and as biocatalyst providing valuable interm...

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Detalhes bibliográficos
Main Authors: Volontè, Federica, Pollegioni, Loredano, Molla, Gianluca, Frattini, Luca, Marinelli, Flavia, Piubelli, Luciano
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2890692/
https://ncbi.nlm.nih.gov/pubmed/20409334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-33
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