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High-throughput assays for DNA gyrase and other topoisomerases
We have developed high-throughput microtitre plate-based assays for DNA gyrase and other DNA topoisomerases. These assays exploit the fact that negatively supercoiled plasmids form intermolecular triplexes more efficiently than when they are relaxed. Two assays are presented, one using capture of a...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2006
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1616945/ https://ncbi.nlm.nih.gov/pubmed/16936317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl504 |
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