Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers

An immunofluorescence assay was developed to identify proteins specifically binding to oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-κB p50 protein was incubated with either bead-bound NF-κB consensus sequence or a bead-bound ODN combinatorial libra...

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Bibliografiske detaljer
Main Authors: Yang, Xianbin, Li, Xin, Prow, Tarl W., Reece, Lisa M., Bassett, Suzanne E., Luxon, Bruce A., Herzog, Norbert K., Aronson, Judy, Shope, Robert E., Leary, James F., Gorenstein, David G.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2003
Fag:
NAR Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC156057/
https://ncbi.nlm.nih.gov/pubmed/12736320
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