Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers

An immunofluorescence assay was developed to identify proteins specifically binding to oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-κB p50 protein was incubated with either bead-bound NF-κB consensus sequence or a bead-bound ODN combinatorial libra...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Yang, Xianbin, Li, Xin, Prow, Tarl W., Reece, Lisa M., Bassett, Suzanne E., Luxon, Bruce A., Herzog, Norbert K., Aronson, Judy, Shope, Robert E., Leary, James F., Gorenstein, David G.
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 2003
Sujets:	NAR Methods Online
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156057/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12736320/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng054
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