Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays

Model single base extension (SBE) genotyping reactions with individual deoxy-, dideoxy- and acyclonucleoside triphosphates are monitored by MALDI-TOF mass spectrometry. Three non-proofreading DNA polymerases display remarkably high misincorporation (up to 64% of correct incorporation) when extending...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Di Giusto, Daniel, King, Garry C.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2003
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149219/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12560510/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng007
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