Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays

Model single base extension (SBE) genotyping reactions with individual deoxy-, dideoxy- and acyclonucleoside triphosphates are monitored by MALDI-TOF mass spectrometry. Three non-proofreading DNA polymerases display remarkably high misincorporation (up to 64% of correct incorporation) when extending...

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Hlavní autoři: Di Giusto, Daniel, King, Garry C.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2003
Témata:
NAR Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC149219/
https://ncbi.nlm.nih.gov/pubmed/12560510
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