Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays

Model single base extension (SBE) genotyping reactions with individual deoxy-, dideoxy- and acyclonucleoside triphosphates are monitored by MALDI-TOF mass spectrometry. Three non-proofreading DNA polymerases display remarkably high misincorporation (up to 64% of correct incorporation) when extending...

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Bibliografische gegevens

Gepubliceerd in:	Nucleic Acids Res
Hoofdauteurs:	Di Giusto, Daniel, King, Garry C.
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Oxford University Press 2003
Onderwerpen:	NAR Methods Online
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149219/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12560510/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng007
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