Protection against Shiga Toxin 1 Challenge by Immunization of Mice with Purified Mutant Shiga Toxin 1

Shiga toxin 1 (Stx1) of enterohemorrhagic Escherichia coli O157:H7 was cloned, and four mutant Stx1s were constructed by site-directed mutagenesis with PCR. The wild-type and mutant Stx1s with amino acid replacements at positions 167 and 170 of the A subunit were purified by one-step affinity chroma...

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Bibliographic Details

Published in:	Infect Immun
Main Authors:	Ishikawa, Satoshi, Kawahara, Kazuyoshi, Kagami, Yutaka, Isshiki, Yasunori, Kaneko, Aki, Matsui, Hidenori, Okada, Nobuhiko, Danbara, Hirofumi
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2003
Subjects:	Microbial Immunity and Vaccines
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC155758/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12761104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.71.6.3235-3239.2003
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