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Pressure-Jump-Induced Kinetics Reveals a Hydration Dependent Folding/Unfolding Mechanism of Ribonuclease A

Pressure-jump (p-jump)-induced relaxation kinetics was used to explore the energy landscape of protein folding/unfolding of Y115W, a fluorescent variant of ribonuclease A. Pressure-jumps of 40 MPa amplitude (5 ms dead-time) were conducted both to higher (unfolding) and to lower (folding) pressure, i...

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Main Authors: Font, J., Torrent, J., Ribó, M., Laurents, D. V., Balny, C., Vilanova, M., Lange, R.
פורמט: Artigo
שפה:Inglês
יצא לאור: Biophysical Society 2006
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גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC1557576/
https://ncbi.nlm.nih.gov/pubmed/16798802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.082552
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