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Pressure versus temperature unfolding of ribonuclease A: An FTIR spectroscopic characterization of 10 variants at the carboxy-terminal site

FTIR spectroscopy was used to characterize and compare the temperature- and pressure-induced unfolding of ribonuclease A and a set of its variants engineered in a hydrophobic region of the C-terminal part of the molecule postulated as a CFIS. The results show for all the ribonucleases investigated,...

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Main Authors: Torrent, Joan, Rubens, Peter, Ribó, Marc, Heremans, Karel, Vilanova, Maria
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2001
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373970/
https://ncbi.nlm.nih.gov/pubmed/11274463
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