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mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

The study of free-living nitrogen-fixing organisms in bulk soil is hampered by the great diversity of soil microbial communities and the difficulty of relating nitrogen fixation activities to individual members of the diazotroph populations. We developed a molecular method that allows analysis of ni...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Bürgmann, Helmut, Widmer, Franco, Sigler, William V., Zeyer, Josef
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154784/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12676666/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.4.1928-1935.2003
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