mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

The study of free-living nitrogen-fixing organisms in bulk soil is hampered by the great diversity of soil microbial communities and the difficulty of relating nitrogen fixation activities to individual members of the diazotroph populations. We developed a molecular method that allows analysis of ni...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Bürgmann, Helmut, Widmer, Franco, Sigler, William V., Zeyer, Josef
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2003
Sujets:	Microbial Ecology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12676666/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.4.1928-1935.2003
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