mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

The study of free-living nitrogen-fixing organisms in bulk soil is hampered by the great diversity of soil microbial communities and the difficulty of relating nitrogen fixation activities to individual members of the diazotroph populations. We developed a molecular method that allows analysis of ni...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Principais autores:	Bürgmann, Helmut, Widmer, Franco, Sigler, William V., Zeyer, Josef
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 2003
Assuntos:	Microbial Ecology
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12676666/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.4.1928-1935.2003
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