mRNA Extraction and Reverse Transcription-PCR Protocol for Detection of nifH Gene Expression by Azotobacter vinelandii in Soil

The study of free-living nitrogen-fixing organisms in bulk soil is hampered by the great diversity of soil microbial communities and the difficulty of relating nitrogen fixation activities to individual members of the diazotroph populations. We developed a molecular method that allows analysis of ni...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Bürgmann, Helmut, Widmer, Franco, Sigler, William V., Zeyer, Josef
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Microbial Ecology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12676666/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.4.1928-1935.2003
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