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Optimization of Single-Base-Pair Mismatch Discrimination in Oligonucleotide Microarrays

The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of...

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Udgivet i:Appl Environ Microbiol
Principais autores: Urakawa, Hidetoshi, El Fantroussi, Said, Smidt, Hauke, Smoot, James C., Tribou, Erik H., Kelly, John J., Noble, Peter A., Stahl, David A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154504/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12732557/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.5.2848-2856.2003
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