Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differi...

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Autores principales: El Fantroussi, Said, Urakawa, Hidetoshi, Bernhard, Anne E., Kelly, John J., Noble, Peter A., Smidt, H., Yershov, G. M., Stahl, David A.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2003
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC154776/
https://ncbi.nlm.nih.gov/pubmed/12676724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.4.2377-2382.2003
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