Optimization of Single-Base-Pair Mismatch Discrimination in Oligonucleotide Microarrays

The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of...

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Detalhes bibliográficos
Main Authors: Urakawa, Hidetoshi, El Fantroussi, Said, Smidt, Hauke, Smoot, James C., Tribou, Erik H., Kelly, John J., Noble, Peter A., Stahl, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC154504/
https://ncbi.nlm.nih.gov/pubmed/12732557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.5.2848-2856.2003
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