Optimization of Single-Base-Pair Mismatch Discrimination in Oligonucleotide Microarrays

The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of...

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Bibliographic Details
Main Authors:	Urakawa, Hidetoshi, El Fantroussi, Said, Smidt, Hauke, Smoot, James C., Tribou, Erik H., Kelly, John J., Noble, Peter A., Stahl, David A.
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology 2003
Subjects:	Methods
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC154504/ https://ncbi.nlm.nih.gov/pubmed/12732557 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.5.2848-2856.2003
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Optimization of Single-Base-Pair Mismatch Discrimination in Oligonucleotide Microarrays

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