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Optimization of Single-Base-Pair Mismatch Discrimination in Oligonucleotide Microarrays

The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of...

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Bibliografske podrobnosti
Main Authors: Urakawa, Hidetoshi, El Fantroussi, Said, Smidt, Hauke, Smoot, James C., Tribou, Erik H., Kelly, John J., Noble, Peter A., Stahl, David A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC154504/
https://ncbi.nlm.nih.gov/pubmed/12732557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.5.2848-2856.2003
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