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A Detergent-Based Assay for the Detection of Promiscuous Inhibitors

At micromolar concentrations, many small molecules self-associate into colloidal aggregates that non-specifically inhibit enzymes and other proteins. Here we describe a protocol for identifying aggregate-based inhibitors and distinguishing them from small molecules that inhibit via specific mechanis...

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Hlavní autoři: Feng, Brian Y, Shoichet, Brian K
Médium: Artigo
Jazyk:Inglês
Vydáno: 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1544377/
https://ncbi.nlm.nih.gov/pubmed/17191086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.77
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