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Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris
The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequential glycosylation reactions that mimic early processing of N-glycans in humans and other higher mammals. After eliminating nonhuman glycosylation by deleting the initiating α-1,6-mannosyltransferase gene from P....
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC154291/ https://ncbi.nlm.nih.gov/pubmed/12702754 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0931263100 |
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