Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants

To investigate the stringency of the Escherichia coli selenocysteine insertion sequence (SECIS) requirements, libraries of SECIS variants were screened via a novel method in which suppression of the selenocysteine (Sec) opal codon was coupled to bacteriophage plaque formation. The SECIS variant libr...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Sandman, Karen E., Tardiff, Daniel F., Neely, Lori A., Noren, Christopher J.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2003
Schlagworte:	Articles
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC153732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12682374/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg304
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