Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants

To investigate the stringency of the Escherichia coli selenocysteine insertion sequence (SECIS) requirements, libraries of SECIS variants were screened via a novel method in which suppression of the selenocysteine (Sec) opal codon was coupled to bacteriophage plaque formation. The SECIS variant libr...

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Опубликовано в::	Nucleic Acids Res
Главные авторы:	Sandman, Karen E., Tardiff, Daniel F., Neely, Lori A., Noren, Christopher J.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2003
Предметы:	Articles
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC153732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12682374/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg304
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