Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants

To investigate the stringency of the Escherichia coli selenocysteine insertion sequence (SECIS) requirements, libraries of SECIS variants were screened via a novel method in which suppression of the selenocysteine (Sec) opal codon was coupled to bacteriophage plaque formation. The SECIS variant libr...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Sandman, Karen E., Tardiff, Daniel F., Neely, Lori A., Noren, Christopher J.
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2003
Assuntos:	Articles
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC153732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12682374/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg304
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