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Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism

Fluorescence resonance energy transfer and fluorescence polarization anisotropy are used to investigate single molecules of the enzyme staphylococcal nuclease. Intramolecular fluorescence resonance energy transfer and fluorescence polarization anisotropy measurements of fluorescently labeled staphyl...

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Autores principales: Ha, Taekjip, Ting, Alice Y., Liang, Joy, Caldwell, W. Brett, Deniz, Ashok A., Chemla, Daniel S., Schultz, Peter G., Weiss, Shimon
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences 1999
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC15321/
https://ncbi.nlm.nih.gov/pubmed/9927664
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