Single-pair fluorescence resonance energy transfer on freely diffusing molecules: Observation of Förster distance dependence and subpopulations

Photon bursts from single diffusing donor-acceptor labeled macromolecules were used to measure intramolecular distances and identify subpopulations of freely diffusing macromolecules in a heterogeneous ensemble. By using DNA as a rigid spacer, a series of constructs with varying intramolecular donor...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Deniz, Ashok A., Dahan, Maxime, Grunwell, Jocelyn R., Ha, Taekjip, Faulhaber, Ann E., Chemla, Daniel S., Weiss, Shimon, Schultz, Peter G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1999
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22352/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10097095/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.7.3670
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