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Single-pair fluorescence resonance energy transfer on freely diffusing molecules: Observation of Förster distance dependence and subpopulations

Photon bursts from single diffusing donor-acceptor labeled macromolecules were used to measure intramolecular distances and identify subpopulations of freely diffusing macromolecules in a heterogeneous ensemble. By using DNA as a rigid spacer, a series of constructs with varying intramolecular donor...

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Detalhes bibliográficos
Main Authors: Deniz, Ashok A., Dahan, Maxime, Grunwell, Jocelyn R., Ha, Taekjip, Faulhaber, Ann E., Chemla, Daniel S., Weiss, Shimon, Schultz, Peter G.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC22352/
https://ncbi.nlm.nih.gov/pubmed/10097095
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