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Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism

Fluorescence resonance energy transfer and fluorescence polarization anisotropy are used to investigate single molecules of the enzyme staphylococcal nuclease. Intramolecular fluorescence resonance energy transfer and fluorescence polarization anisotropy measurements of fluorescently labeled staphyl...

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Main Authors: Ha, Taekjip, Ting, Alice Y., Liang, Joy, Caldwell, W. Brett, Deniz, Ashok A., Chemla, Daniel S., Schultz, Peter G., Weiss, Shimon
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 1999
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC15321/
https://ncbi.nlm.nih.gov/pubmed/9927664
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