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Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase

Bacteriophage T7 gp4 serves as a model protein for replicative helicases that couples deoxythymidine triphosphate (dTTP) hydrolysis to directional movement and DNA strand separation. We employed single-molecule fluorescence resonance energy transfer methods to resolve steps during DNA unwinding by T...

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Bibliografiska uppgifter
Huvudupphov: Salman Syed, Manjula Pandey, Smita S. Patel, Taekjip Ha
Materialtyp: Artigo
Språk:Inglês
Utgiven: Elsevier 2014-03-01
Serie:Cell Reports
Länkar:http://www.sciencedirect.com/science/article/pii/S2211124714001223
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