Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning

Transformation-associated recombination (TAR) cloning in yeast is used to isolate a desired chromosomal region or gene from a complex genome without construction of a genomic library. The technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TA...

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Autores principales: Leem, Sun-Hee, Noskov, Vladimir N., Park, Jung-Eun, Kim, Seung Il, Larionov, Vladimir, Kouprina, Natalay
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2003
Materias:
NAR Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC152883/
https://ncbi.nlm.nih.gov/pubmed/12626728
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