A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast

<p>Abstract</p> <p>Background</p> <p>Transformation-associated recombination (TAR) cloning in yeast is a unique method for selective isolation of large chromosomal fragments or entire genes from complex genomes. The technique involves homologous recombination, during yeast spheroplast transformation...

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Bibliografische Detailangaben

Hauptverfasser:	Barrett J Carl, Ouspenski Ilia, Leem Sun-Hee, Kouprina Natalay, Noskov Vladimir N, Larionov Vladimir
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	BMC 2003-04-01
Schriftenreihe:	BMC Genomics
Schlagworte:	transformation-associated recombination cloning gene isolation counter-selection
Online-Zugang:	http://www.biomedcentral.com/1471-2164/4/16
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