A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast

BACKGROUND: Transformation-associated recombination (TAR) cloning in yeast is a unique method for selective isolation of large chromosomal fragments or entire genes from complex genomes. The technique involves homologous recombination, during yeast spheroplast transformation, between genomic DNA and...

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Autori principali: Noskov, Vladimir N, Kouprina, Natalay, Leem, Sun-Hee, Ouspenski, Ilia, Barrett, J Carl, Larionov, Vladimir
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2003
Soggetti:
Methodology Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC156606/
https://ncbi.nlm.nih.gov/pubmed/12720573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-16
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