Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes
<p>Abstract</p> <p>We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted into a microbe vector. This method includes rolling-circle phi29 amplification (RCA) of the sequence <it>in vitro </it>an...
محفوظ في:
| المؤلفون الرئيسيون: | , , , |
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| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
BMC
2011-10-01
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| سلاسل: | Biological Procedures Online |
| الوصول للمادة أونلاين: | http://www.biologicalproceduresonline.com/content/13/1/8 |
| الوسوم: |
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